Comparative proteomics analysis reveals the molecular mechanisms
underlying the accumulation difference of bioactive constituents in
Taxilli Herba from two hosts
Abstract
Taxilli Herba (TH) is famous for its semi-parasitic feature, with
hundreds of host species. The molecular mechanisms governing the
accumulation of bioactive constituents in TH from different hosts have
yet to be completely understood. The purpose of this study was to
investigate the differences in synthesis and accumulation of bioactive
constituents in TH from Morus alba L. and Liquidambar formosana Hance.
The proteome of TH from two hosts were compared, and proteins were
identified using tandem mass tag (TMT) technology. Sixteen bioactive
constituents were quantitatively determined and analyzed in conjunction
with the results of proteomics. A total of 5681 proteins were detected,
of which 259 were significantly up-regulated and 274 significantly
down-regulated in expression levels. Differentially expressed proteins
(DEPs) were shown to be mostly engaged in cellular process and metabolic
process by GO analysis. DEPs were considerably abundant in amino acid
metabolic pathways, according to KEGG pathway analysis. The changes in
the contents of 16 bioactive constituents were mostly compatible with
the expression patterns of relevant structural proteins. The results of
this study will provide basic information for the role of protein in the
secondary metabolism biosynthesis pathway and the molecular mechanism of
the quality formation of TH.