This study presents a novel approach in nanomedicine for targeted cancer therapy by leveraging folic acid-receptor (FR) interactions and reactive oxygen species (ROS)-responsive nanocarriers. Utilizing big data to advance nanomedicine, we developed an innovative amphiphilic conjugate, folic acid (FA) decorated dextran-block-poly copolymer (FA-Dex-b-PPS), designed to serve as ROS-responsive nanocarriers specifically for prostate cancer treatment. The chemical structure of FA-Dex-b-PPS was confirmed via Fourier transform infrared spectroscopy and proton nuclear magnetic resonance. The self-assembly into ROS-responsive nanoparticles and subsequent degradation were characterized through fluorescence spectroscopy, dynamic light scattering, and transmission electron microscopy. Therapeutic nanocarriers encapsulating doxorubicin (Dox) were prepared via dialysis, demonstrating efficient oxidant-triggered Dox release in vitro. Cytotoxicity assays revealed high biocompatibility, with cell survival rates exceeding 85% at 400 μg/mL. Confocal laser scanning microscopy confirmed the efficient internalization of FA-Dex-b-PPS-Dox nanoparticles by PC3 cells via FR-mediated endocytosis, surpassing non-targeted Dex-b-PPS-Dox nanoparticles. Furthermore, in vitro and in vivo xenograft mouse model analyses consistently demonstrated that FA-Dex-b-PPS-Dox nanoparticles exhibited superior anti-tumor efficacy against PC3 cells compared to non-targeted and free Dox counterparts. These findings underscore the potential of tumor-targeted, ROS-responsive nanocarriers in enhancing the efficacy of cancer therapy.

The serve burn or scald wounds always face persistent infections and self-repair function decline caused by serious tissue necrosis, leading to delayed healing or even sepsis. In this work, we proposed a click-chemistry hydrogel delivery system of antibacterial and tissue remodeling function nanovesicles for deep scald wound treatment. An hydrophilic photodynamic aggregation-induced emission photosensitizer 4-(2-(5-(4-(diphenylamino)phenyl)thiophen-2-yl)vinyl)-1-(2-hydroxyethyl) pyridin-1-ium bromide (THB) was firstly encapsulated into nanovesicles derived from easily accessible adipose stem cells (ANVs) to create THB@ANVs, which exhibits enhanced bacteria-targeting property and multiple tissue remodeling effects. To deliver THB@ANVs, an injectable click-chemistry hydrogel of carboxymethyl chitosan was used to form a wound treatment system for deep scald wounds. The hydrogel can well matching the wound morphology and respond to the acidic microenvironment of the wound to accelerate sustained release. In vivo wound healing evaluations show that the composite hydrogel can efficiently accelerate wound healing by reducing the number of bacteria, promoting early angiogenesis, and regulating immune reaction. This study provides a simple, low-cost, and effective “one-stop” strategy with diverse functions and wide applicability for scald wound remodeling and antibiosis.