IntroductionMultiple causes could alter liver functions including infections, biliary disorders, viral or non-viral hepatitis, autoimmunity, and drugs. Brucellosis, zoonotic infection, presents with unspecific varied signs and symptoms affecting multiple organs (1). This infection is widely prevalent in both animals and humans (2). The liver is commonly involved in both acute and chronic cases of Brucellosis. When infected, patients may experience a slight increase in transaminase levels and mild swelling of both the liver and spleen. Occasionally, acute hepatitis can occur, but it’s usually not the only symptom of infection (3). Conjugated hyperbilirubinemia along with normal liver transaminase, serum alkaline phosphatase (ALP), and jaundice highly suggest Dubin-Johnson syndrome (DJS). Patients less likely present with mild abdominal pain, pruritus, nausea, or vomiting. DJS is a rare, chronic mainly autosomal recessive disease caused by mutations in the gene encoding for proteins involved in hepatobiliary transport of non-bile salt organic anions, leading to conjugated hyperbilirubinemia (4). Mutations in the multidrug resistance-associated protein-2 (MRP-2) gene are responsible for this disorder (4). The diagnosis of this benign condition is important to avoid unnecessary anxiety or intervention (5). Herein, for the first time, we report a case of a 35-year-old man presenting with jaundice for the first time in his life caused by brucella hepatitis, that lead to the diagnosis of concomitant DJS.