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An in-depth coho salmon (Onchorhynchus kisutch) ovarian follicle proteome reveals coordinated changes across diverse cellular processes during the transition from primary to secondary growth
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  • Emma Timmins-Schiffman,
  • Jennifer Telish,
  • Chelsea Field,
  • Christopher Monson,
  • Jose Guzman,
  • Brook Nunn,
  • Graham Young,
  • Kristy Forsgren
Emma Timmins-Schiffman
University of Washington

Corresponding Author:[email protected]

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Jennifer Telish
California State University Fullerton
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Chelsea Field
California State University Fullerton
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Christopher Monson
University of Washington
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Jose Guzman
University of Washington
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Brook Nunn
University of Washington
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Graham Young
University of Washington
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Kristy Forsgren
California State University Fullerton
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Abstract

Teleost fishes are a highly diverse and ecologically essential group of aquatic vertebrates and include coho salmon, Onchorhynchus kisutch. Coho are semelparous and all ovarian follicles develop synchronously. Owing to their ubiquitous distribution, teleost provide critical sources of food worldwide through subsistence, commercial fisheries, and aquaculture. Enhancement of commercial hatchery practices requires a detailed knowledge of teleost reproductive physiology. Despite decades of research on teleost reproductive processes, an in-depth proteome of teleost ovarian development has yet to be generated. We describe a coho salmon ovarian proteome of over 5700 proteins, generated with data independent acquisition, revealing the suite of detectable proteins that change through the transition from primary to secondary ovarian follicle development. This transition is critical for puberty onset, egg quality, and further embryonic development. Primary ovarian follicle development was marked by differential abundances of proteins involved in carbohydrate metabolism, protein turnover, and the complement pathway, suggesting elevated metabolism as the oocytes enter maturation. The greatest proteomic shift occurred during the transition from primary to secondary follicle growth, with increased abundance of proteins underlying cortical alveoli formation, extracellular matrix reorganization, iron binding, and cell-cell signaling. This work provides a foundation for identifying biomarkers of salmon oocyte stage and quality.
23 Sep 2024Submitted to PROTEOMICS
25 Sep 2024Submission Checks Completed
25 Sep 2024Assigned to Editor
25 Sep 2024Review(s) Completed, Editorial Evaluation Pending
25 Sep 2024Reviewer(s) Assigned
12 Nov 2024Editorial Decision: Revise Minor
20 Nov 2024Review(s) Completed, Editorial Evaluation Pending
20 Nov 20241st Revision Received
21 Nov 2024Editorial Decision: Accept