Control of plant virus disease largely depends on the induced plant defense achieved by the external application of synthetic chemical inducers with the ability to modify defense-signaling pathways. However, most of the molecular mechanisms underlying these chemical inducers remain unknown. Here, we developed a lentinan-loaded hy-drogel with a core-shell structure and discovered how it protects plants from different virus infections. The hydrogel was synthesized by adding a chitosan shell on the sur-face of the polyanion sodium alginate-calcium ion-lentinan (LNT) hydrogel (SL-gel) to form CSL-gel. CSL-gel exhibits the capacity to prolong the stable release of lenti-nan and promote calcium ions release. Application of CSL-gel on the root of plants induces broad-spectrum resistance against TMV, TuMV, PVX and TRV. Further-more, RNA-seq analysis identified that the calmodulin-like protein 19 gene (CML19) is upregulated by the sustained release of calcium ions from the CSL-gel, and silenc-ing and overexpression of CML19 alter the susceptibility and resistance of tobacco to TMV. Our findings provide evidence that the novel and synthetic CSL-gel with the sustainable release of LNT and calcium ion strongly inhibits the plant virus infection. This study uncovers a novel mode of action by which CSL-gel with the stable release of calcium ion triggers CML19 expression.